ARIZONA CENTER FOR FERTILITY STUDIES

		The Medical Team
			Robert W. McGaughey, Ph.D. EDUCATION Augustana College B.A. 1963 University of Colorado M.A. 1965 Boston University Ph. D. 1968 AREAS OF SPECIALIZATION Developmental Biology, Reproductive Biology. Regulation of mammalian oocyte maturation. In Vitro Fertilization and Embryo Transfer. PROFESSIONAL EMPLOYMENT Worcester Foundation for Experimental Biology, Research Associate, 1965-1968 Worcester Foundation for Experimental Biology, Staff Scientist, 1968-1969 Lalor Foundation (Cambridge, England), Postdoctoral Fellow, 1969-1970 University of Colorado Research Associate, 1970-1971 Arizona State University Assistant Professor, 1971-1974 Arizona State University Associate Professor, 1974-1980 University of Colorado (Sabbatical Leave) Visiting Professor, 1978 Arizona State University Professor, 1980- Arizona Center for Fertility Studies Laboratory Consultant, 1984- Arizona State University Sabbatical Leave, 1985 Arizona State University Sabbatical Leave, 1991 PROFESSIONAL ORGANIZATIONS Society of Developmental Biology American Society for Cell Biology American Fertility Society AWARDS AND HONORS Teaching Fellowship, University of Colorado, 1963-1965 Tri-Beta Biological Honorary Lalor Foundation Postdoctoral Fellowship, 1969-1970 Whitehall Foundation Research Grant, 1979-1981 ARDCO Research Contract, 1987-1988 RESEARCH GRANTS AND AWARDS (Past Five Years) Role of the Cytoskeleton on Trophectoderm Development NICHD, $329,178 1988-1991 Research in Infertility Good Samaritan Health Services $54,000 1990-1995 Interaction of anti-HIV drugs with placental models NIH, $303,985 1991-1995 PEER REVIEWS OF RESEARCH GRANT APPLICATIONS Department of Agriculture, Competitive Research Grants Office (ad hoc reviewer). Site Visit Team, NIH Center Grant Review Committee. PEER REVIEWS OF RESEARCH ARTICLES FOR PUBLICATION Developmental Biology Biology of Reproduction Cell Biology Proceedings of the National Academy of Sciences Gamete Research Journal of Reproduction and Fertility Human Reproduction PUBLICATIONS McGaughey, R.W. and J.C. Daniel, Jr. 1966. Effects of estradiol-17ß on fertilized eggs in vitro. J. Reprod. Fertil. 11:325-33 McGaughey, R.W., J.H. Marston and M.C. Chang. 1968. Fertilizing life of mouse sperm in the female tract. J. Reprod. Fert. 16:147-150. McGaughey, R.W. and M.C. Chang. 1969. Meiosis of mouse eggs before and after sperm penetration. J. Exp. Zool. 170:397-409 McGaughey, R.W. and M.C. Chang. 1969. Inhibition of fertilization and production of heteroploidy in eggs of mice treated with colchine. J. Exp. Zool. 171:465-480. Harper, M.J.K., M.C. Chang, R. Skarnes and R.W. McGaughey. 1970. Effect of antilymphocytic serum (ALS) on pregnancy in rodents. Biol. Reprod. 2:335-345. McGaughey, R.W. and M.C. Chang. 1970. X-irradiation of the pregnant Mongolian gerbil. Anat. Rec. 136:37-53. McGaughey, R.W. and M.C. Chang. 1971. Chromosomes at prometaphase and metaphase of the first cleavage in mouse and hamster eggs. J. Exp. Zool. 177:31-39. McGaughey, R.W. and C. Polge. 1971. Cytogenic analysis of pig oocytes matured in vitro. J. Exp. Zool. 176:383-396 McGaughey, R.W. and F.A. Murray. 1972. Properties of blastokinin: Amino acid composition, evidence for subunits, and estimation of isoelectric point. Fertil. Steril. 23:399-404 Murray, F.A., R.W. McGaughey and M.J. Yarus. 1972. Blastokinin: Its size, shape, and an indication of the existence of its subunits. Fertil. Steril. 23:69-75. McGaughey, R.W. and M.C. Chang. 1973. Initial chromosomal lesions induced by X-irradiating primary spermatocytes of mice. Can. J. Genet. Cytol. 15:341-348 McGaughey, R.W. 1975. A comparison of fluids from small and large follicles of the pig. Biol. Reprod. 13:147-153. McGaughey, R.W. 1977. The culture of pig oocytes in minimal medium and the influence of progesterone and estradiol-17ß on meiotic maturation. Endocrinology. 100:39-45. McGaughey, R.W. and J. Van Blerkom. 1977. Patterns of polypeptide synthesis of porcine oocytes during maturation in vitro. Devel. Biol. 56:241-254. McGaughey, R.W. 1977. The maturation of porcine oocytes in minimal, defined culture media with varied macromolecular supplements and varied osmolarity. Exptl. Cell Res. 109:25-30 Van Blerkom, J. and R.W. McGaughey. 1978. Molecular differentiation of the rabbit ovum: I. During oocyte maturation in vivo and in vitro. Devel. Biol. 63:139-150. Van Blerkom, J. and R.W. McGaughey. 1978. Molecular differentiation of the rabbit ovum: II. During the preimplantation development of in vivo and in vitro matured oocytes. Devel. Biol. 63:151-164. McGaughey, R.W., D.H. Montgomery and J.D. Richter. 1979. Germinal vesicle configurations and patterns of polypeptide synthesis of porcine oocytes from antral follicles of different size, as related to their competency for spontaneous maturation. J. Exp. Zoology. 290:239-254 Richter, J.D. and R.W. McGaughey. 1979. Specificity of inhibition by steroids of porcine oocyte maturation in vitro. J. Exp. Zoology. 290:81-90. Rice, C. and R.W. McGaughey. 1980. Effect of testosterone and dibutyryl cAMP on the spontaneous maturation of pig oocytes. J. Reprod. Fertil.. 62:245-256. Richter, J.D. and R.W. McGaughey. 1979. Patterns of polypeptide synthesis in mouse oocytes during germinal vesicle breakdown and during maintenance of the germinal vesicle stage by dibutyryl cAMP. Devel. Biol. 83:188-192. Fleming, A.D. and R.W. McGaughey. 1982. A progesterone-binding component in porcine ovarian follicular fluid. J. Endocrinol. 94:69-76. Racowsky, C. and R.W. McGaughey. 1982. Further studies of the effects of follicular fluid and membrana granulosa cells on the spontaneous maturation of pig oocytes. J. Reprod. Fertil. 66:505-512. Racowsky, C. and R.W. McGaughey. 1982. In the absence of protein, estradiol suppresses meiosis of porcine oocytes in vitro. J. Exp. Zool. 224:103-110. McGaughey, R.W. 1985. Amino acids can induce nondisjunction during maturation of mammalian oocytes in vitro. J. Exp. Zool. 234:160-165. Capco, D.G. and R.W. McGaughey. 1986. Cytoskeletal reorganization during early mammalian development: Analysis using embedment-freesections. Devel. Biol. 115:115-458. Homa, S.T., C. Racowsky and R.W. McGaughey. Lipid analysis of immature pig oocytes. J. Reprod. Fertil. 77:425-434. Nemiro, J.S. and R.W. McGaughey. 1986. An alternative to IVF-ET: The successful transfer of human oocytes and spermatozoa to distal oviduct. Fertil. Steril. 46:644-652. McGaughey, R.W. and J.S. Nemiro. 1987. Correlation of estrogen levels with oocytes aspirated and with pregnancy in a program of clinical tubal transfer. Fertil. Steril. 48:98-106. Homa, S.T., R.W. McGaughey and C. Racowsky. 1988. Isolated subpopulations of mass-harvested pig oocytes and their characterization by size, incidence of atresia and competence to mature in culture. Journal of Reprod. Fert. 82:81-86. McGaughey, R.W. and J.S. Nemiro. 1988. Initial success with establishment of pregnancy by tubal transfer (GIFT). Infertility 11:233-243. McGaughey, R.W. and D.G. Capco. 1989. A unique cytoskeletal element in mammalian eggs: Cytological and molecular evidence for its composition. Cell Motility and the Cytoskeleton. 13:104-111. Webster, S. and R.W. McGaughey. 1990. The cortical cytoskeleton and its role in sperm penetration of the mammalian egg. Devel. Biol. 142:60-74. McGaguhey, R.W., C. Racowsky, V. Rider, K. Baldwin, A.A. DeMarais and S. Webster. 1990. Ultrastructural correlates of meiotic maturation in mammalian oocytes. J. Electron Micro. Techn. 16:257-280. Gallicano, G.I., R.W. McGaughey and D.G. Capco. 1991. Cytoskeleton of the mouse egg and embryo: Reorganization of planar elements. Cell Motility and the Cytoskeleton. 18:143-154. Gallicano, G.I., R.W. McGaughey and D.G. Capco. 1992. Cytoskeletal sheets appear as universal components of mammalian eggs. J. Experimental Zoology. 263:194-203. Cacpo, D.G., G. I. Gallicano, R.W. McGaughey, K.H. Downing, and C.A. Larabell. 1993. Cytoskeletal sheets of mammalian eggs and embryos: A lattice-like network of intermediate filaments. Cell Motility and the Cytoskeleton. 24:85-99 Gallicano, G.I., R.W. McGaughey and D.G. Capco. 1993. Ontogeny of the cytoskeleton during mammalian oogenesis. Micro. Research and Technique. 27:134-144. Gallicano, G.I., S.M. Schwarz, R.W. McGaughey and D.G. Capco. 1993. Protein kinase C, a pivotal regulator of hamster egg activation, functions after elevation of intracellular free calcium. Developmental Biology 156:94-106. Gallicano, G.I., C.A. Larabell, R.W. McGaughey and D.G. Capco. 1994. Novel cytoskeletal elements in mammalian eggs are composed of a unique arrangement of intermediate filaments. 45:211-226. Gallicano, G.I., R.W. McGaughey and D.G. Capco. 1995. Protein Kinase M, the cytosolic counterpart of protein kinase C, remodels the internal cytoskeleton of the mammalian egg during activation. Developmental Biology 167:482-501. Schwarz, S.M., G. I. Gallicano, R.W. McGaughey, D.G. Capco. 1995. A role for intermediate filaments in the establishment of the primitive epithelia during, mammalian embryogenesis. Mechanisms of Develop. 53:305-321. Gallicano, G.I. R.W. McGaughey and D.G. Capco. 1997. Activation of Protein Kinase C after Fertilization is Required for Remodeling the Mouse Egg into the Zygote. Molecular Reprod. And Develop. 46:587-601. CHAPTERS/REVIEWS Chang, M.C., S. Pickworth and R.W. McGaughey. 1969. Experimental hybridization and chromosomes of hybrids. In: Comparative Mammalian Cytogenetics. Ed., Kurt Benirschke, Springer-Verlag, New York, pp. 132-145. McGaughey, R.W. 1978. In vitro maturation of mammalian oocytes. In: Methods in Mammalian Embryology (J.C. Daniel, Ed.) Second Edition. McGaughey, R.W. 1983. Regulation of oocyte maturation. In: Oxford Reviews of Reproductive Biology (Ed., C. Finn), Oxford University Press, Volume 5, pp. 106-130. McGaughey, R.W. 1984. Association of oocytes with follicular cells, and oocyte chromatin during maturation. In: Ultrastructure of Reproduction (Eds., J. Van Blerkom and P.M. Motta), Martinus Nijhoff Publishers, pp. 156-165. ABSTRACTS Chang, M.C., M.J.K. Harper, R.W. McGaughey and R. Skarnes. 1968. Effects of antilymphocytic serum on pregnancy in rodents. Proc. 6th Intern. Conf. On Animal Reprod., Paris (Abstract). McGaughey, R.W., J.H. Marston and M.C. Chang. 1968. Fertilizing life of mouse sperm in the female tract. Fed. Proc. 27, No. 2 (abstract). McGaughey, R.W., and M.C. Chang. 1969. Chromosome behavior at prometaphase and metaphase of the first cleavage in mouse and hamster eggs. Anat. Rec. 163:340 (Abstract). Harper, M.J.K. and R.W. McGaughey. 1970. The inhibition of cell division in the eggs of rabbits by cytochalasin-B in vitro and the reversal of this inhibition in vivo and in vitro. Anat. Rec., proc. 83rd Ann. Session of the Am. Assoc. of Anat. (Abstract). McGaughey, R.W. and J.C. Daniel, Jr.. 1972. Protein patterns of ovarian follicular fluids from pigs. Bio. Reprod. 7:128 (Abstract). Gray, T.M. and R.W. McGaughey. 1975. Protein patterns of mouse uterine fluids. J. Arizona Acad. Sci. 10:30 (Abstract). McGaughey, R.W. 1976. Resumption of pig oocyte maturation in chemically defined minimal culture media. Ninth Ann. Meeting, Soc. Study Reprod., Univ. Penna. (Abstract No. 122). Richter, Joel and R.W. McGaughey. 1978. The influence of estradiol-17ß and of other steroids on porcine oocytes during maturation in chemically defined media. Biol. Reprod. Suppl. No. 1, 18 (20A) (Abstract). Nemiro, J.S. and R.W. McGaughey. 1986. Low tubal transfer: An alternative to in vitro fertilization and embryo transfer. Pacific Coast Fertility Society Annual Meeting, San Diego, April 6-9 (Abstract). Nemiro, J.S. and R.W. McGaughey. 1986. Improved success with tubal transfer (TT) of human oocytes and spermatozoa. Ann. Meeting. The American Fertil. Soc., September 27-30 (Abstract). Nemiro, J.S. and R.W. McGaughey. 1987. Tubal transfer of oocytes and sperm as a successful treatment for infertility. Ann. Clin. Meeting of the Am. Coll. Obst. Gynecol. April 27-30 (Abstract.) McGaughey, R.W. and J.S. Nemiro. 1987. Influence of follicle number, number of oocytes aspirated and quality of aspirated oocytes on successful treatment of infertility by tubal transfer. West Coast Fertil. Society. Palm Springs. (Abstract). Nemiro, J.S. and R.W. McGaughey. 1988. Pregnancy with Tubal Transfer as influenced by method of ovarian stimulation. West Coast Fertility Society Annual Meeting. April, 1988. (Abstract). Mutchler, D.V., and McGaughey, R.W. 1988. Cytoskeletal organization during early development in the mouse. J. Cell Biol. 107, 605a. (Abstract). Webster, S.D. and R.W. McGaughey. 1988. Cytoskeletal interactions between the sperm and the egg at penetration in the Syrian hamster. J. Cell Biol. 107, 178a. (Abstract). McGaughey, R.W. and J.S. Nemiro. 1990. Elevated estrogen influences incidences of oocyte atresia, pregnancy and pregnancy loss with GIFT. West Coast Fertility Society Annual Meeting. April, 1990. (Abstract) Gallicano, G.I., D.G. Capco, and R.W. McGaughey. 1990. Cytoskeletal sheets comprised of intermediate filaments : Novel cytoskeletal elements characteristic of mammalian embryos. J. Cell Biol. 111, 481a (Abstract) Gallicano, G.I., D.G. Capco, and R.W. McGaughey. 1990. The cytoskeleton of the mouse egg and embryo: A role for intermediate filaments. J. Cell Biol. 111, 481a. (Abstract) Gallicano, G.I., C.A. Larabell, R.W. McGaughey, and D.G. Capco. 1991. Substructure of cytoskeletal sheets: A highly organized array of 10 nm filaments with periodic crossbridges. J. Cell Biol. 115, 53a. (Abstract) Larabell, C.A., D.G. Capco, G.I. Gallicano, R.W. McGaughey, K. Dierksen, and K.H. Downing. 1992. Three-dimensional examination of the cytoskeletal sheets of mammalian eggs. Proc. 50th Annual Meeting of the Electron Microscopy Society of America, pp. 914-915. (Abstract) G.I. Gallicano, S.M. Schwarz, R.W. McGaughey, and D.G. Capco. 1992. Protein Kinase C acts in concert with calcium to mediate mammalian egg activation. 32nd Annual Meeting, The American Society for Cell Biology, abstract 95. R.W. McGaughey, V. Usaha, G.I. Gallicano, D.G. Capco and B.L.. Jacobs. 1992. Azidothymidine (AZT), a viral reverse transcriptase inhibitor, disrupts early development in mice. 32nd Annual Meeting, The American Society for Cell Biology, abstract H27. Gallicano, G.I., R.W. McGaughey and D.G. Capco. 1993. Cytoskeletal sheet reorganization at fertilization is controlled by PKM, the cytosolic form of PKC. 33rd Annual Meeting, The American Society for Cell Biology, abstract 1447. Schwarz, S.M., R.W. McGaughey and D.G. Capco. 1993. A role for intermediate filaments on the establishment of the primitive epithelia during mammalian embryogenesis. 33rd Annual Meeting, The American Society for Cell Biology, abstract 1448. Wattier, R.D., V. Usaha, G.I. Gallicano, S.T. Shors, D.G. Capco, B.L. Jacobs and R.W. McGaughey. 1993. Preimplantation development in mice is enhanced and lethal effects of AZT decreased by mouse interferon-ß through induction of an interferon-specific protein. 33rd Annual Meeting, The American Society for Cell Biology, abstract H26. Gallicano, G.I., S.M. Schwarz, R.W. McGaughey, and D.G. Capco. 1994. Cytoskeletal sheets in mammalian eggs and early embryos provide an assembled form of intermediate filaments used later in development. 34th Annual Meeting, The American Society for Cell Biology, abstract 2050. V. Usaha, R.D. Wattier, and R.w> McGaughey. 1995. Oocytes of rodents mature in the presence of an inhibitor of histone h1 kinase but not in the presence of H-89, an inhibitor of other kinases. 35th Annual Meeting, The American Society for Cell Biology, abstract 525. (back to medical team index)